MSE Research Project Database

Protocol development for assays of bone and cartilage tissues from mouse tibio-femoral joints


Project Leader: Kathryn Stok
Staff: Kathryn Stok, Zihui Li
Primary Contact: Kathryn Stok (kathryn.stok@unimelb.edu.au)
Keywords: arthritis; cartilage; health and bioinformatics
Disciplines: Biomedical Engineering
Domains: Convergence of engineering and IT with the life sciences

The Integrative Cartilage Research Group at the University of Melbourne researches links between microstructure, mechanics and biological signalling networks.

Glycosaminoglycans, collagen, DNA, and mineral content are just some of the markers used to understand biological tissue quality. Reproducibly extracting and measuring these markers from small structures (e.g. mouse knees) can be challenging. In this work, we are developing robust in vivo protocols that will form the foundation for future research projects.

The ICR Group is developing, refining, and using biomedical engineering tools and concepts, to explore and understand the effects of mechanics on living musculoskeletal systems on the molecular, cellular, and organ level of organisation, while maintaining a philosophy of respect and compassion for all human and animal life.

The project involves monitoring of biosignals and biomarkers in biological constructs and in vivo animal models. The work requires a creative approach to developing new protocols for evaluating biological and mechanical signalling networks in tissues. Students involved in the project have a Master degree in molecular biology, biotechnology, bioengineering, or a related field with a strong interest in technology and medicine.